Characterization of the salivary microbiome in healthy Thai children

Objective: To investigate the composition of the salivary microbiome of 50 healthy Thai children. Methods: A total 76 provinces in Thailand are grouped into 5 geographical clusters based on unique economics, foods and lifestyles. Geographical locations and the results of an oral assessment were also considered. Genomic DNA was extracted from stimulated sdiva samples. Subsequently, amplicon libraries were prepared by 16S Metagenomic Sequencing Library Preparation. The amplicons were sequenced using an Illumina Miseq platform followed by bioinformatics and statistical analyses. Results: The correlation between oral hygiene status and caries history varied from r

Caries with dental fluorosis and oral health behaviour among 12-year school children in moderate-fluoride drinking water community in Quetta, Pakistan

Objective: To determine the prevalence of dental caries and its relationship with dental fluorosis, oral health behavior and dietary behaviour among 12-year school children in moderate-fluoride drinking water community in Quetta, Pakistan

Study Design: Cross-sectional study

Place and Duration of Study: Government and private schools of Quetta, from November 2012 to February 2013

Methodology: A total of 349 children aged 12-year from 14 randomly selected schools were included. The data collection was done on questionnaire designed for children. Dental caries status was examined by using WHO criteria

Results: Dental caries was found in 81 children [23.2%] with mean DMFT 0.61. Boys had 1.6 times more chance to have dental caries than girls. Dental fluorosis was found in 63.6% of children with majority of moderate degree [50.5%]. Dental fluorosis status was found significantly associated with dental caries status in children. The children, who had mild, moderate and severe fluorosis, had 4 times more chances to develop caries than those who did not have fluorosis. There was no significant association between children's caries status and use of paste, brushing habit, miswak, and visit to the dentist. The use of pastries and juices had a direct relation with the children's dental caries status

Conclusion: Dental caries in children of Quetta is not so much frequent as compared to the fluoride deficient countries. However, the high prevalence of moderate dental fluorosis and consumption of pastries and juices resulted in dental caries